Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Vatica diospyroides symington type LS root extract induces antiproliferation of KB, MCF-7 and NCI-H187 cell lines

Theera Srisawat¹ , Parinuch Chumkaew¹, Kanyanatt Kanokwiroon^2,3, Potchanapond Graidist^2.3, Yaowapa Sukpondma⁴

¹Faculty of Science and Industrial Technology, Prince of Songkla University Suratthani campus, Muang, Suratthani 84000; ²Department of Biomedical Sciences, Faculty of Medicine; ³The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University; ⁴Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai campus, Songkhla 90110 Thailand.

For correspondence:- Theera Srisawat Email: theera.s@psu.ac.th

Received: 2 August 2014 Accepted: 28 April 2015 Published: 29 June 2015

Citation: Srisawat T, Chumkaew P, Kanokwiroon K, Graidist P, Sukpondma Y. Vatica diospyroides symington type LS root extract induces antiproliferation of KB, MCF-7 and NCI-H187 cell lines. Trop J Pharm Res 2015; 14(6):961-965 doi: 10.4314/tjpr.v14i6.4

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the therapeutic efficacy of V. diospyroides Symington type LS root extract as a chemopreventive agent against various cancer cell lines.

Methods: Acetone root extract was evaluated for in vitro cytotoxicity against KB (oral cavity cancer), MCF-7 (breast cancer), and NCI-H187 (small cell lung cancer), using Resazurin microplate assay (REMA). Toxicity against a representative normal cells, Vero (African green monkey kidney), was assessed using green fluorescence protein (GFP)-based assay.

Results: V. diospyroides root extract showed significant cytotoxic effects on KB and MCF-7 cell lines in a dose-dependent manner with IC₅₀ of 35.05 ± 1.45 and 36.63 ± 3.40 µg/mL, respectively. NCI-H187 was not significantly inhibited (≤ 19.39 % inhibition) at the concentrations tested. IC₅₀ against Vero cells was outside the concentration range of 0.2 - 50 µg/mL.

Conclusion: These results indicate that the root extract of V. diospyroides has in vitro cytotoxic effect on human oral cavity cancer and breast cancer cells. No toxic effect on normal cells was observed. Thus, the extract may provide bioactive substances for human cancer therapy.

Keywords: Breast cancer, Oral cavity cancer, Lung cancer, Cytotoxicity, Vero cells, Vatica diospyroides